Description.6% cross-linked agarose beads conjugated via thioether bond to nanoCLAMP P2808 for single-step purification with native elution of SMT3 (S. cerevisiae SUMO) and human SUMO3 from crude lysates. SBC > 10 mg (235 nmol)/ml resin. Fusions can be cleaved from resin using Ulp1.
Binding capacity. >10 mg (235 nmol) SUMO-GFP fusion per ml resin.
Chemical resistance. Retains 100% of binding activity after >20 cycles with 10 min. contact time with 0.1 M NaOH or after 2 hr. in 100% DMF.
